Extended Data Fig. 5: IL-10-expressing HER2 CAR-T cells exhibit an expanded subpopulation of CD8+ terminally exhausted T cells with upregulated gene expression encoding cell cycling, cytotoxicity, and OXPHOS. | Nature Biotechnology

Extended Data Fig. 5: IL-10-expressing HER2 CAR-T cells exhibit an expanded subpopulation of CD8+ terminally exhausted T cells with upregulated gene expression encoding cell cycling, cytotoxicity, and OXPHOS.

From: IL-10-expressing CAR T cells resist dysfunction and mediate durable clearance of solid tumors and metastases

Extended Data Fig. 5: IL-10-expressing HER2 CAR-T cells exhibit an expanded subpopulation of CD8+ terminally exhausted T cells with upregulated gene expression encoding cell cycling, cytotoxicity, and OXPHOS.The alternative text for this image may have been generated using AI.

a, The experimental setting and timeline is described in Fig. 4. b, Heatmap representing the expression levels of representative marker genes in each cluster. c, Projection of IL-10 HER2 CAR-T cells and HER2 CAR-T cells into a reference atlas of tumor-infiltrating lymphocytes. Reference atlas states are indicated as colored cells. CD8+ terminally-exhausted T cell cluster was characterized as high expression of granzymes, multiple inhibitory receptors (Pdcd1, Ctla4, Lag3, Tigit, Havcr2/TIM-3, etc.) and Tox16. Shown are cell subtype compositions for each group. Black contour lines represent the density of projected cells. d, Gene signature scores for each group of samples (HER2 CAR-T and IL-10 HER2 CAR-T cells) for the indicated gene sets.

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