Extended Data Fig. 4: A mutation in the linker improves recombination efficiency of Brec1278-Zif268. | Nature Biotechnology

Extended Data Fig. 4: A mutation in the linker improves recombination efficiency of Brec1278-Zif268.

From: Activation of recombinases at specific DNA loci by zinc-finger domain insertions

Extended Data Fig. 4: A mutation in the linker improves recombination efficiency of Brec1278-Zif268.The alternative text for this image may have been generated using AI.

Plasmid-based activity assay for Brec1278-Zif268 containing a mutation in the right linker (Brec1-(GGS)8-Zif268-(GGS)6-GRS-GGS) compared to Brec1278-Zif268 (Brec1-(GGS)8-Zif268-(GGS)8) on the loxBTR and loxBTR-5-zif (A) target sites. The test was performed at 100 ug/ml L-arabinose. Activity of the wild-type Brec1 is shown as a control. Recombination efficiencies were calculated from ratios of recombined and non-recombined band intensities. The assays were performed three times (n = 3 biologically independent replicates). The upper band represents the unrecombined plasmid (line with two triangles), and the lower band represents the recombined plasmid (line with one triangle). M = Marker.

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