Extended Data Fig. 10: Amino acid sequence and recombination activity of RecFlex.
From: Activation of recombinases at specific DNA loci by zinc-finger domain insertions
a The amino acid sequence of RecFlex aligned to Cre (one-letter code) is shown. Dots indicate conserved residues; asterisks denote stop codons. b Quantification of the recombination rates for RecFlex and RecFlex-Zif268 fusion complex on five loxFlex sites, genomic loxMECP2 target site, and their lox-zif versions, in which the lox-sites were flanked by the Zif268 binding motifs. Recombination efficiencies were calculated from ratios of recombined and non-recombined band intensities shown in Fig. 6c. The assay was performed in triplicates (n = 3), plotted as dots. The bar graphs represent mean values, the error bars indicate the standard deviation from the mean. Statistical relevance of the triplicates was assessed using an unpaired two-sided t-test. P-values are indicated.
