Extended Data Fig. 8: WGCNA and pseudotime analyses of integrated snRNA-seq datasets. | Nature Biotechnology

Extended Data Fig. 8: WGCNA and pseudotime analyses of integrated snRNA-seq datasets.

From: Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain

Extended Data Fig. 8: WGCNA and pseudotime analyses of integrated snRNA-seq datasets.

a. WGCNA dendrogram of gene modules constructed from astrocytes across different time points. b. Dot plot shows the expression levels of astrocyte gene modules across major cell type. c. WGCNA dendrogram of gene modules constructed from immature and UL excitatory neurons across different time points. d. Dot plot shows the expression levels of neuron gene modules in each major cell type. e. Co-expression plot of the top 25 hub genes (left) and UMAP plot (right) of module hub gene expression score for neuron gene modules M1. f. Violin plot of harmonized module score of neuron gene module M1 in UL excitatory neurons across different time points. Centerline, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range; points, outliers. Wilcoxon test (two-sided, ****, p < 0.0001; reference group, 5m_Org). g. UMAP dimensionality reduction displays the pseudotime trajectories of neurons from the integrated snRNA-seq. Each cell is colored by its pseudotime trajectory assignment. Pseudotime analysis separated by time point. One-sided Kolmogorov–Smirnov test (****, p < 2.2e-16; reference group, 5m_Org).

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