Extended Data Fig. 10: CD38 mediates inflammation-induced metabolic and mitochondrial stresses in human astrocytes. | Nature Biotechnology

Extended Data Fig. 10: CD38 mediates inflammation-induced metabolic and mitochondrial stresses in human astrocytes.

From: Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain

Extended Data Fig. 10: CD38 mediates inflammation-induced metabolic and mitochondrial stresses in human astrocytes.

a. Immunostaining of in vitro glia-enriched cortical organoids at day 0, day 1 and day 2 post-TNFα treatment: astrocytes (hCD38, green; CD44 cyan), chemokines (CXCL10, magenta). Scale bars, 50 μm. b. Quantitative PCR analyses of selected genes in organoids (CTRL = 4; TNFα1d = 4; TNFα2d = 6). Each dot represents one organoid. Bars, mean ± s.d. c. Flow cytometry sorting strategy for GFAP::tdTomato+ cells. d. Quantitative PCR analyses of selected genes in sorted astrocytes (CTRL = 3 independent experiments; TNFα1d = 3 independent experiments). Each dot represents one independent experiment. Bars, mean ± s.d. Two-sided t-test, *p = 0.013555, ***p = 0.000948.

Source data

Back to article page