Extended Data Fig. 1: Representative examples of paDSF screening results.
From: Protein-adaptive differential scanning fluorimetry using conformationally responsive dyes
For full dye screen results for all proteins, including comparisons of raw paDSF curves collected for each protein with different dyes, see data availability statement. (a) Results of a dye screen against the SYPRO-incompatible protein nuclear-hormone receptor 23. Far left panel: representative unsuccessful DSF conducted with SYPRO Orange. Right panels: examples of successful paDSF data, collected with six different paDSF dyes. Plots show dyes at four different concentrations, demonstrating a dose-responsive relationship between RFU and dye concentration. (b) Representative examples of resolution of four archetypes of SYPRO Orange incompatibility by paDSF dyes. (c) Examples of successful paDSF data collected for four additional proteins. As in panel A, dyes are plots show dyes at four different concentrations, demonstrating a dose-responsive relationship between RFU and dye concentration.
