Extended Data Fig. 4: Branched poly(A) tails bind PABPC1 protein.
From: Branched chemically modified poly(A) tails enhance the translation capacity of mRNA
(a) Electrophoretic mobility shift assay of three branched poly(A) oligo with varying concentrations of GST-tagged recominant human PABPC1 protein (PABPC1-GST). The stem oligo was labeled with Alexa Fluore 546 at 5′end. Both stem and branching poly(A)’s were 30 nt and modified with PS-2MOE at the last six bases and ddC at the 3′ end. (b) Gel shift assay of the same modified poly(A) oligo without branching. Gels are representative of at least two experiments.
