Extended Data Fig. 3: Gating strategies.
From: Discovery of tumor-reactive T cell receptors by massively parallel library synthesis and screening
(a) Gating strategy for the isolation of CD4+ and/or CD8+ T cells from patient tumor material, for the purpose of identifying patient-derived TCRs by single-cell TCR sequencing (relating to the patient TCR libraries used in Figs. 2 to 4). (b) Gating strategy for the isolation of activated reporter T cells in TCR library screens (relating to Figs. 2b–d, 3a,d and 4a, and Extended Data Figs. 7a–c, and 9b,d. (c) Gating strategy for T cell activation assays (relating to Figs. 3c,e,f and 4b,c, and Extended Data Figs. 7d–f and 9a. CD137 served as activation marker when using primary T cells, and CD69 served as activation marker when using Jurkat cells. (d) Gating strategy for T cell cytotoxicity assays (relating to Figs. 3g and 4d).
