Extended Data Fig. 4: Quality control of the NKIRTIL063 TCR library.
From: Discovery of tumor-reactive T cell receptors by massively parallel library synthesis and screening
(a) Schematic overview of the custom ‘2-reads-on-1-strand’ sequencing method. (b) Overall assembly accuracies and frequencies of TCRs in the fully assembled NKIRTIL063 VCV library (n = 935 TCRs). Dots represent individual TCRs. Box plots depict the median, the interquartile range and whiskers extending to minimal and maximal values. (c) Pie chart depicting the overall fraction of NKIRTIL063 VCV molecules with and without sequence errors (taking both assembly accuracy and sequence mutations into account). (d) The fraction of NKIRTIL063 library TCRs successfully expressed at the cell surface of library-transduced cells was determined by isolation of mouse TCRβ+ Jurkat cells by flow cytometry, followed by deep sequencing. NKIRTIL063 library-expressing Jurkat cells that were not subjected to cell sorting were used as reference. Internal control TCRs DMF4 and DMF5 are highlighted for reference.
