Extended Data Fig. 5: Quality control of the OVC190 TCR libraries.
From: Discovery of tumor-reactive T cell receptors by massively parallel library synthesis and screening
(a) Overall assembly accuracies and frequencies of TCRs in the fully assembled OVC190 CD4+ TIL-derived VCV libraries (combined n = 1,341 TCRs). Selected TCRs were encoded in replicate, resulting in a total of 2,899 unique sequences that were assembled in two separate reactions (SP1 and SP2). Dots represent individual TCRs. Box plots depict the median, the interquartile range and whiskers extending to minimal and maximal values. (b) Overall assembly accuracies and frequencies of TCRs in the fully assembled OVC190 CD8+ TIL-derived VCV library (n = 274 TCRs). (c) Pie chart depicting the overall fraction of OVC190 CD4+ and CD8+ TIL-derived VCV molecules with and without sequence errors (taking both assembly accuracy and sequence mutations into account).
