Extended Data Fig. 5: TCR-MAP accurately identifies Treg TCR reactivities.
(a) Mouse genome-wide TCR-MAP screen results of PAD4-reactive 6MNO TCR+ SrtA-Jurkats. 56-aa peptide fragments covering the murine proteome were expressed in H2-IAb+ CIITA+CTSS+ G5-target cells SrtA-Jurkat cells were added to achieve a final effector-to-target ratio of 1:1. Each dot represents one peptide with the y axis reporting the negative log10 p adjusted values by Mageck and the x axis indicating the geometric mean of the enrichment of the peptide across three replicates. Fold enrichment is defined as the ratio of the abundance of the peptide in the sorted population relative to the input library. Peptides highlighted in red indicate validated hits from the screen. PAD4, peptidylarginine deiminase 4.
