Extended Data Fig. 1: Design of an inducible SrtA expression construct for TCR-MAP. | Nature Biotechnology

Extended Data Fig. 1: Design of an inducible SrtA expression construct for TCR-MAP.

From: High-throughput discovery of MHC class I- and II-restricted T cell epitopes using synthetic cellular circuits

Extended Data Fig. 1

(a) Vector designs for the different inducible NFAT-SrtA reporters tested. (b) Jurkat cells transduced with the various NFAT-SrtA reporters were stimulated for 6 h (top) or 1, 4, 6, 8, 24 h (bottom) with either 1 μg/ml anti-CD3 antibody (top & bottom) or PMA/I (top). Percentage of murine CD40L expressed on the cell surface of Jurkats was assessed by flow cytometry. Error bars indicate the mean and SD. Data in b are representative of n = 3 independent biological replicates. PMA, phorbol 12-myristate 13-acetate. I, ionomycin.

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