Extended Data Fig. 5: The RuvC domain is responsible for the trans-cleavage activity of SpyCas9.
From: Trans-nuclease activity of Cas9 activated by DNA or RNA target binding
a-c, Denaturing gel showing target dsDNA cis-cleavage (a), target ssDNA cis-cleavage (b), and non-specific ssDNA trans-cleavage (c) by SpyCas9 wild-type (WT), RuvC-dead mutant (D10A) or HNH-dead mutant (H840A). dsDNA and ssDNA substrates were labeled with Cy5 or Cy3. Assays in Extended Data Fig. 5 were repeated three times independently with similar results.
