Extended Data Fig. 9: Comparison of structures of SpyCas9-sgRNA-target ssDNA and SpyCas9-sgRNA-target ssRNA.
From: Trans-nuclease activity of Cas9 activated by DNA or RNA target binding
a, Structural comparison between the SpyCas9-sgRNA-target ssDNA (PDB: 4OO8) complex and the SpyCas9-sgRNA (PDB: 4ZT0) complex. Vector length correlates with the domain motion scale. b, Structural comparison between the SpyCas9-sgRNA-target ssRNA complex and the SpyCas9-sgRNA complex (PDB: 4ZT0). c, Superposition of the SpyCas9-sgRNA-target ssRNA complex onto the SpyCas9-sgRNA-target ssDNA complex. The structure of ssDNA bound state is colored in silver. Protein domains of SpyCas9-sgRNA-target ssRNA are colored according to Fig. 4g. d, Structural overlay of the little variation domains in ssRNA bound state and ssDNA bound state complexes. e, Structural overlay of the domains that underwent marked conformational change in ssRNA bound state and ssDNA bound state complexes. f, Structural overlay of nucleic acids in ssRNA bound state and ssDNA bound state complexes. g, Superposition of the guide RNA (orange)-target ssRNA (megenta) duplex onto an A-form RNA duplex (gray).
