Extended Data Fig. 3: Verification of metalloporphyrin production by HRMS and biocatalytic synthesis of Zincphyrin.
From: High-yield porphyrin production through metabolic engineering and biocatalysis
a-d, Identification of Co-CPIII, Ni-CPIII, Cu-CPIII, and Mn-CPIII by high-resolution mass spectrometry (HRMS). All the accurate mass measurements for impurities were within < 5 ppm of the theoretical m/z value. e, HPLC profile of the enzymatic catalysis of CPIII for the in vitro synthesis of Zincphyrin using HemHSa and zinc sulfate. The reaction is conducted in Tris-HCl buffer (pH 8.0) at 37 °C under the following conditions: i) In the reaction system, only 50 μM CPIII is present. ii) In condition i, 50 μM ZnSO4 and 1 μM HemHSa were added.
