Fig. 1: Wobble base pairs modulate RNA editing in an orientation-dependent manner.
From: Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA
a, Design of the cis-acting editing reporter and illustration of the applied triplet base-pairing motifs. The orientation of wobble base pairs at nearest neighbor positions or the presence of G or C counter bases modulate Δ-editing at the central A of a triplet in comparison to its fully Watson–Crick base-paired counterpart. All triplet base-pairing motifs with ocher background color were installed at the empty dotted outline within the cis-acting editing construct to generate the results in b,c. b, Suppression of RNA editing in different target triplets using G•U wobble base pairs or G•A mismatches. c, Enhancing RNA editing in different target triplets using U•G wobble base pairs. The Sanger sequence analysis in b and c was performed after transfection of editing reporter plasmids into ADAR1 p110 Flp-In T-REx cells. Data in b and c are shown as the mean ± s.d. of n = 3 biological replicates. For statistical analysis, a Student’s t-test (two-tailed, parametric) was applied.
