Extended Data Fig. 2: G·U wobbles improve efficiency and precision of trans-acting LEAPER guide RNAs for endogenous targets.
From: Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA
(a-b) Editing heat-map of the LEAPER guide RNA binding site within the indicated transcripts. The basic design column, LEAPER, lacks G·A mismatches and wobble base pairs. The other guide RNAs contain either G·A mismatches or G·U wobbles at G·U amenable sites or a combination of both solutions at all bystander sites. In the latter case, G·A mismatches are placed at sites not amenable to G·U wobbles. The triplet context for each listed editing event is given with the target adenosine highlighted in green and all off-target adenosines in blue. The position of each site is given relative to the transcript and the target adenosine (±0 position). The editing events detected at position 2546 ( + 47) and 2548 ( + 49) of the NUP43 transcript were natural events and were thus skipped in regard to bystander suppression. Editing was performed with plasmid-borne guide RNA and endogenous target transcripts in HEK293FT cells (endogenous ADAR). Data are shown as the mean editing percentage ± s.d. of N = 3 biological replicates.
