Extended Data Fig. 6: Intraoperative SICTERS-based Raman image-guided surgery for orthotopic mouse colon tumor by BBT NPs.
From: Self-stacked small molecules for ultrasensitive, substrate-free Raman imaging in vivo
Mice bearing orthotopic CT26-Luc tumor were divided into three groups. PBS, mice received PBS without the tumor resection. Conventional surgery, mice received 40 mg kg−1 of BBT NPs, followed by the tumor resection with naked eyes after 24 h. Image-guided surgery, mice received 40 mg kg−1 of BBT NPs, followed by the intraoperative Raman image-guided tumor resection after 24 h. a, The intraoperative Raman imaging-guided tumor resection. White dotted circles indicated the primary tumor. Raman image and histologic analysis with H&E staining showed primary tumor (region 1, black dotted circle) and microtumor/metastatic tumor (region 2, black dotted circle). No tumor cells remained in the surgical margin (regions 3 and 4) after the intraoperative SICTERS-based Raman imaging-guided surgery. b, Conventional surgery of tumors by naked eyes. Histologic analysis with H&E staining confirmed the primary tumor (region 1, black dotted circle) and residual tumors in the surgical margin (regions 2 and 3, black dotted circles). Image shown is representative of n = 5 independent replicates of experiments with similar results (a,b). c, Kaplan–Meier survival curves of mice. n = 5 biologically independent mice. Kaplan–Meier survival curves were analyzed using log-rank (Mantel–Cox) test, with P values indicated on the graphs. P < 0.05 was considered statistically significant. ns denotes no significant difference. d, Histological analysis of the resected gastrointestinal tract and tumor or cecum of mice at the end of different treatments in (c). Black dotted circles indicated the tumor. Upper bars, 10 mm; lower bars, 1 mm. Image shown is representative of n = 5 independent replicates of experiments with similar results.
