Extended Data Fig. 8: In vivo depth analysis of SICTERS imaging by covering BBT NPs-bearing ALNs with porcine skin slices.
From: Self-stacked small molecules for ultrasensitive, substrate-free Raman imaging in vivo
a, Illustration of the experimental setup. BBT NPs (80 μg of BBT) were injected though the left front paw of mice. Fifteen minutes after injection, ALNs were exposed and then covered with different number of porcine skin slices for imaging. The thickness of each piece of porcine skin slice was 0.4 mm. b, The SICTERS-based Raman imaging of BBT NPs-bearing ALNs, covered with porcine skin slices with thicknesses of 0 mm, 0.4 mm, 0.8 mm, 1.2 mm, and 1.6 mm, respectively. c, Raman spectra of different regions in (b). Blue column, peaks at 894 cm−1. Raman measurement was carried out with a 5 × objective, 830-nm laser excitation, a laser power of 62.6 mW, acquisition time of 1 s and one time accumulation.
