Extended Data Fig. 8: Sorting of SARS-CoV-2 RBD-specific human ASCs. | Nature Biotechnology

Extended Data Fig. 8: Sorting of SARS-CoV-2 RBD-specific human ASCs.

From: Rapid discovery of monoclonal antibodies by microfluidics-enabled FACS of single pathogen-specific antibody-secreting cells

Extended Data Fig. 8

(a) Comparison of IgG signal originating from cryopreserved ASCs on d7 post vaccination in BG-agarose beads incubated with (+VHH) or without VHH–SNAP (−VHH; different experiment than shown in Fig. 3). The plots show 1,139 (−VHH) and 1,289 (+VHH) events at 2% contour level. (b) Comparison of RBD-specific signal of cryopreserved ASCs on d7 post vaccination in BG-agarose beads incubated with (+VHH, secreted antibodies) or without VHH–SNAP (−VHH, surface antibodies). The plots show 1,139 (−VHH) and 1,289 (+VHH) events at 2% contour level. (c) Representative gating strategy for identifying RBD-specific human ASCs (gated as live/CD20−/CD38+/IgM−/IgG+/RBD+) and comparison of fresh and cryopreserved samples (day 8 post vaccination). Cells in hydrogels could be easily identified by forward and side scatter; therefore, the gating is different compared to other experiments.

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