Extended Data Fig. 5: Engraftment of blood cells from PB1.1 BFP iPSCs in cohort 3 transplant recipients. | Nature Biotechnology

Extended Data Fig. 5: Engraftment of blood cells from PB1.1 BFP iPSCs in cohort 3 transplant recipients.

From: Long-term engrafting multilineage hematopoietic cells differentiated from human induced pluripotent stem cells

Extended Data Fig. 5

(a) Scatter dot plot correlating human cells in the bone marrow (BM) with the interval of retinoid (R) treatment during differentiation (shown as days). Each circle represents one animal, colour coded to represent myeloid, myelo-lymphoid, and erythro-myelo-lymphoid multilineage (MLE) patterns of engraftment. Number of mice receiving each duration of retinoid during differentiation (n) is shown. Number of unengrafted (NEG) mice indicated. Error bars, mean ± s.e.m. Data from 4CH 3B5A and 4CH 30 A mesoderm inductions was pooled. Flow cytometry profiles from (b) bone marrow (BM) and (c) spleen (SPL) of a multilineage repopulated recipient (mouse (m)410). (b) Erythroid cells (GYPA+CD43+) were enriched in the BFP low (lo) BM fraction. BFP high (hi) cells included erythroid cells (GYPA+ CD45−), CD19+ B cells, CD33+ and CD13+ myeloid cells, and CD45+CD34+CD38lo/− HSCs. (c) The spleen also contained erythroid cells (GYPA+CD45-) and CD19+CD45+ B cells.

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