Fig. 5: Robust hematopoietic engraftment with cells differentiated using protocol 3.

a–d, Engraftment of BM and SPL in transplant recipients of RM TOM (a), PB1.1 BFP (b), PB5.1 (c) and PB10.5 (d) cells showing the phenotype of engrafting cells and the level of engraftment. Error bars, mean ± s.e.m. e–h, Tissue distribution of engrafting cells in MLE recipients of RM TOM (e), PB1.1 BFP (f), PB5.1 (g) and PB10.5 (h) cells in BM, SPL, THY and PB at 12 and 16 weeks. Error bars, mean ± s.e.m. i, Flow cytometry analysis of BM in engrafted mice for each cell line showing GYPA⁺ erythroid lineage and CD45⁺ lymphoid and myeloid cells. j, BM, SPL and THY or mediastinal lymph node (LN) tissue of RM TOM-engrafted mouse m574, showing GYPA⁺ erythroid, CD45⁺CD19⁺ B cell, CD45⁺CD3⁺ T cell, CD45⁺CD33⁺/CD13⁺ myeloid and CD45⁺CD34⁺CD38^lo/- stem cell populations in the BM, CD45⁺sIgM⁺ B cells and CD45⁺CD3⁺ T cells in the SPL and THY or mediastinal LN tissue containing CD45⁺CD3⁺CD4⁺ and CD45⁺CD3⁺CD8⁺ T cells and a population of CD45⁺CD19⁺ B cells.

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