Extended Data Fig. 4: Probing the involvement of eIF3D-dependent translation initiation by dual-capped mRNA.
From: Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation
(a) Probing involvement of eIF3D-dependent translation initiation using eIF3D knockdown or eIF4E/eIF4G interaction inhibitor. (b ~ d) Expression of mono-capped RLuc and dual-capped FLuc mRNA co-transfected in wild-type or eIF3D-knockdown HeLa cells by siRNA. n = 3, biological replicates. Mean ± sem. P values were calculated by two-sided unpaired t-test. (e ~ g) In vitro translation of mono-capped FLuc and dual-capped NLuc with or without 4EGI-1 in rabbit reticulocyte lysates (RRL). n = 3, biological replicates. Mean ± sem. P values were calculated by two-sided unpaired t-test.
