Extended Data Fig. 9: Quantification of SARS-CoV-2-RBD-specific T cells in mice after vaccination. | Nature Biotechnology

Extended Data Fig. 9: Quantification of SARS-CoV-2-RBD-specific T cells in mice after vaccination.

From: Chemical and topological design of multicapped mRNA and capped circular RNA to augment translation

Extended Data Fig. 9: Quantification of SARS-CoV-2-RBD-specific T cells in mice after vaccination.The alternative text for this image may have been generated using AI.

(a) Gating strategy for single and viable T cells in splenocytes. CD4+ or CD8+ T-effector memory (Tem) cells (CD44+CD62L−) were further analyzed to detect the expression of cytokines stimulated by corresponding RBD peptide pools (b ~ c) Representative flow plots for specific CD4+ T cell response (b) or CD8+ T cell response (c). (d ~ h) Measurement of the level of IL-2 (d), IL-4 (e), IL-13 (f), TNF (g) or IFN-γ (h) in the supernatants of peptide pool-stimulated splenocytes with ELISA. n = 4, biological replicates. Mean ± sem. P values were calculated by unpaired two-sided t-test for comparison within each treatment conditions (DMSO vs S-peptide). P values were calculated by one-way ANOVA with Šídák’s multiple comparisons test for comparison between constructs under S-peptide treatment conditions.

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