Extended Data Fig. 9: Comparing cost and synthesis time of pegRNAs with different production methods. | Nature Biotechnology

Extended Data Fig. 9: Comparing cost and synthesis time of pegRNAs with different production methods.

From: Rapid generation of long, chemically modified pegRNAs for prime editing

Extended Data Fig. 9: Comparing cost and synthesis time of pegRNAs with different production methods.The alternative text for this image may have been generated using AI.

a. Urea-PAGE analysis of S-pegRNA for 17 bp insertion at the HEK3 locus. b. Urea-PAGE analysis of three-fragment ligation products for 40 bp insertion at the HEK3 locus (234 bp). M, marker. c. Comparative analysis of cost and production period between four S-pegRNAs and their corresponding L-pegRNAs. d. Cost and production period for L-pegRNAs and L-epegRNAs of varied lengths across different synthesis scales, with or without HPLC purification.

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