Extended Data Fig. 8: Heatmap showing osimertinib resistance scores of 1,817 protein variants in the presence of a co-occuring T790M mutation.

These variants were generated by prime editing in exons 18-24 of EGFR in PC-9 cells containing the T790M mutation. Boxes outlined in yellow and gray indicate protein variants causing resistant and intermediate phenotypes, respectively. The numbers at the bottom of each heatmap represent the location in the EGFR amino acid sequence. At each position, the amino acid in the reference sequence is shown at the top. Ninety-four protein variants with P-values of two-sided Fisher’s exact test greater than 0.05 or odds ratios lower than 3 were excluded from the analysis and are shown with a white background. Protein variants for which no pegRNAs were designed are shown as gray boxes.