Fig. 2: SEED-Selection can simultaneously enrich for edits at multiple loci.
From: SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci
a–c, T cells were edited with TRAC and B2M RNPs (+M3814) and transduced with TRAC-CAR SEED and B2M-CD47 SEED HDRTs. Edited cells were then immunomagnetically purified with anti-B2M and anti-TCR (n = 2 donors). a, Multiplexed editing and enrichment strategy. b, Representative flow cytometry plots of TCR, CAR, B2M and CD47 expression. c, Percentage of B2M+ or TCR+ cells and fully edited cells (TCR−CAR+B2M−CD47+). –d–g 1.6 × 108 T cells were edited with TRAC and B2M RNPs and transduced with TRAC-CAR SEED and B2M-CD47 SEED HDRTs using a GMP-compatible workflow (n = 2 donors). d, Overview of large-scale editing workflow. e, Expansion of cells after multiplexed editing. f, Number and composition of edited cells after expansion. g, Representative flow cytometry plots of TCR, CAR, B2M and CD47 expression. FITC, fluorescein isothiocyanate.
