Extended Data Fig. 3: Evaluation of TRAC intron and exon targeted gRNAs.
From: SEED-Selection enables high-efficiency enrichment of primary T cells edited at multiple loci
a, Percentage of TCR– CAR– cells after editing with TRAC RNP alone or RNP with CAR HDRT (n = 3 donors). Data displayed as mean plus SEM. Significance was assessed with a two-sided paired t test. b, Comparison of TRAC-intron and exon-targeted HDRTs encoding a CAR and EGFRt. c–g, T cells were edited with TRAC intron or TRAC exon-targeted RNPs and HDRTs (b). Edited cells from each condition were then immunomagnetically purified with anti-TCR (n = 2 donors). c, Flow cytometry plots of TCR and CAR (anti-mouse F(ab’)2) expression d, Histograms of CAR expression in TCR– CAR+ cells. e, Median fluorescence intensity (MFI) of CAR expression in TCR– CAR+ cells. f, Percentage of TCR– CAR+ cells in purified (blue) and non-purified samples (grey). g, Relative enrichment of fully edited cells after purification: (% of purified sample) / (% of non-purified sample).
