Extended Data Fig. 4: Roles of different DNA exonucleases in TALED-mediated A-to-G editing in mtDNA.
From: Leveraging base excision repair for efficient adenine base editing of mitochondrial DNA
a, C-to-T editing frequencies induced by DdCBE at the ND1 and ND4 sites in wild-type (WT) and hUNG/hSMUG1 double-knockout (hUNG/hSMUG1_DKO) 293FT cells. b, A-to-G editing frequencies induced by sTALED at the ND1 and ND4 sites in WT and hAPE1 knockout (hAPE1_KO) 293FT cells. c, A-to-G editing frequencies induced by sTALED at the ND1 and ND4 sites in WT, hExo1 knockout (hExo1_KO), hExoG knockout (hExoG_KO) and hDNA2 knockout (hDNA2_KO) 293FT cells. d, Schematic diagram of the scenario in which hMGME1-mediated DNA strand excision destroys the binding region for TadA8e-containing half of sTALED and therefore no A-to-G editing is induced. e, Schematic diagram of the scenario in which DddA mediates C-to-U editing at both DNA strands in the spacer region and therefore DNA double strand breaks (DSBs) are generated. f, Schematic diagram of the scenario in which the previous round of A-to-G editing (red base pair) occurred in one DNA strand (dark green strand) within the spacer region and therefore a new round of A-to-G editing (orange base) can occur in the other DNA strand (light green strand). For a-c, the data are presented as the mean ± s.d. from three independent experiments.
