Extended Data Fig. 10: Characterization of the editing efficiency, nuclear genome-wide mutation and cellular toxicity induced by engineered TALEDs.

a, Schematic of the constructions of sTALED, esTALED6 and esTALED6R targeting mtDNA. b, Heatmaps of A-to-G editing frequencies induced by the indicated editors. The sequences of the TALE-binding regions are in blue, and bystander editing can be found in the boxes with dashed black lines. The boxes with dashed blue lines represent the narrowed editing windows. c and d, Number of A/T-to-G/C (c) and C/G-to-T/A (d) nuclear genome-wide substitutions induced by the indicated editors. e and f, Cell viability was measured by MTS assay at day 2 (e) and day 4 (f) post-transfection after treated with the indicated editors targeting ND1 site. For b, e and f, the data are presented as the mean ± s.d. from three independent experiments. For c and d, the eGFP data are presented as the mean ± s.d. from two independent experiments, sTALED, esTALED6 and esTALED6R are presented as the mean ± s.d. from three independent experiments. Statistical analysis was performed via two-tailed Student’s t-test. For e and f, statistical analysis was performed via two-tailed Welch’s t-test.