Extended Data Fig. 1: TALED, but not DdCBE, induced bystander editing in TALE-binding regions at other target sites.
From: Leveraging base excision repair for efficient adenine base editing of mitochondrial DNA
a, Schematic of the constructions of sTALED and DdCBE targeting ND4 site. NTD, N-terminal domain of TALE array; CTD, C-terminal domain of TALE array; MTS, mitochondrial targeting sequence; UGI, uracil glycosylase inhibitor. b, A-to-G editing frequencies induced by sTALED and C-to-T base editing frequencies induced by DdCBE at the ND4 site in 293FT cells. NT, non-transfected control. c, Schematic of the constructions of sTALED and DdCBE targeting ND5.3 site. d, A-to-G editing frequencies induced by sTALED and C-to-T base editing frequencies induced by DdCBE at the ND5.3 site in 293FT cells. The sequences of TALE-binding regions are in blue and bystander editing can be found in the boxes with dashed line. The data are presented as the mean ± s.d. from three independent experiments.
