Extended Data Fig. 10: Additional data plot formats.

(a) Varied scale boxplots of three spring wheat varieties DM Ceibo, MS INTA 415 and Saeta grown 2018–2022 in wet and dry years at two dose rates of DMNB-T6P in 2018, 2020 and three dose rates in 2021, 2022 compared to control with no DMNB-T6P. p < 0.0001, 0.065, 0.0348, 0.010 for 2018, 2020, 2021, 2022, respectively. Each data point represents an individual field plot. Median is shown as box line. (b) Varied scale boxplots of grain number per m2 in response to DMNB-T6P spray over four seasons. Three spring wheat varieties DM Ceibo, MS INTA 415 and Saeta grown 2018–2022 at two dose rates of DMNB-T6P in 2018, 2020 and three dose rates of DMNB-T6P in 2021, 2022 compared to control with no DMNB-T6P. Each data point represents an individual plot. Median is shown as box line. (c) Variable scale boxplots Individual grain weight (mg) in response to DMNB-T6P spray over four seasons. Three spring wheat varieties DM Ceibo, MS INTA 415 and Saeta grown 2018–2022 at two dose rates of DMNB-T6P in 2018, 2020 and three dose rates of DMNB-T6P in 2021, 2022 compared to control with no DMNB-T6P. Each data point represents an individual plot. Median is shown as box line. (d) Variable scale boxplots as equivalent plot to A, also showing mean (red diamond) and the Standard Error of Difference (SED) for comparisons between pairs of means for combinations of genotype and treatment. (e) Variable scale boxplots as equivalent plot to B, also showing mean (red diamond) and SED for comparisons between pairs of means for combinations of genotype and treatment. (f) Variable scale boxplots as equivalent plot to C, also showing mean (red diamond) and SED for comparisons between pairs of means for combinations of genotype and treatment. (g) Mean grain yield (kg ha⁻¹) from four field experiments over four separate occasions (years), showing overall means for each T6P treatment (black dots and lines) and means for each genotype for each T6P treatment (red dot – Ceibo, blue dot – INTA415, green dot – Saeta). SEDs shown for comparisons between pairs of overall T6P treatment means (SED type A) and for comparisons between pairs of means for combinations of genotype and T6P treatment (SED type B), based on 34, 24, 33, and 33 degrees of freedom for the 2018, 2020, 2021 and 2021 experiments, respectively. For all experiments except 2018 the replication levels are the same for all treatments, but for the 2018 experiment the T6P plots were only measured for 5 of the 6 replicate blocks – the SED A shown (145.6) is for comparing the means for the two T6P doses, with a smaller SED (139.4) for comparing the mean for the Control with that for either of the T6P doses; the SED B shown (252.1) is for comparing means for two treatments with this lower replication level (that is comparisons between genotypes at either T6P dose, or between doses for each genotype), with smaller SEDs for comparing the mean for the Control treatment with that for either dose for each genotype (241.4) and for comparing means for genotypes for the Control treatment (230.2). (h) Mean grain number per m² from four field experiments over four separate occasions (years), showing overall means for each T6P treatment (black dots and lines) and means for each genotype for each T6P treatment (red dot – Ceibo, blue dot – INTA415, green dot – Saeta). SEDs shown for comparisons between pairs of overall T6P treatment means (SED type A) and for comparisons between pairs of means for combinations of genotype and T6P treatment (SED type B), based on 34, 24, 33, and 33 degrees of freedom for the 2018, 2020, 2021 and 2021 experiments, respectively. For all experiments except 2018 the replication levels are the same for all treatments, but for the 2018 experiment the T6P plots were only measured for 5 of the 6 replicate blocks – the SED A shown (333.7) is for comparing the means for the two T6P doses, with a smaller SED (319.5) for comparing the mean for the Control with that for either of the T6P doses; the SED B shown (578.0) is for comparing means for two treatments with this lower replication level (that is comparisons between genotypes at either T6P dose, or between doses for each genotype), with smaller SEDs for comparing the mean for the Control treatment with that for either dose for each genotype (553.4) and for comparing means for genotypes for the Control treatment (527.6). (i) Mean individual grain weight (mg per grain) from four field experiments over four separate occasions (years), showing overall means for each T6P treatment (black dots and lines) and means for each genotype for each T6P treatment (red dot – Ceibo, blue dot – INTA415, green dot – Saeta). SEDs shown for comparisons between pairs of overall T6P treatment means (SED type A) and for comparisons between pairs of means for combinations of genotype and T6P treatment (SED type B), based on 34, 24, 33, and 33 degrees of freedom for the 2018, 2020, 2021 and 2021 experiments, respectively. For all experiments except 2018 the replication levels are the same for all treatments, but for the 2018 experiment the T6P plots were only measured for 5 of the 6 replicate blocks – the SED A shown (0.441) is for comparing the means for the two T6P doses, with a smaller SED (0.422) for comparing the mean for the Control with that for either of the T6P doses; the SED B shown (0.764) is for comparing means for two treatments with this lower replication (that is comparisons between genotypes at either T6P dose, or between doses for each genotype), with smaller SEDs for comparing the mean for the Control treatment with that for either dose for each genotype (0.731) and for comparing means for genotypes for the Control treatment (0.697). Box plots a-f range from the first (Q1) to the third quartile (Q3) of the distribution and contain the 25th to 75th percentiles of dataset, respectively, representing the interquartile range (IQR). The centre line inside each box represents the median value (50th percentile). Whiskers extending below Q1 and above Q3 denote minimum and maximum values of the dataset within 1.5 x IQR from the 25th and 75th percentiles, respectively.