Extended Data Fig. 1: Formation of ELP-protein nanoparticle.
From: Self-assembling protein nanoparticles for cytosolic delivery of nucleic acids and proteins
a) PAGE gel image depicting result of sortase-mediated Cre-LPETG conjugation to V1-ELP at varying ratios. The reaction solution underwent centrifugal filtration against a 100 kDa filter to remove unreacted Cre. b) Quantification of Cre-V1-ELP conjugation efficiency via densitometry analysis, measured as percentage of total ELP conjugated to Cre. c) Diameter of Cre-V1-ELP nanoparticles (n = 3 technical replicates). d) PAGE gel image depicting result of sortase-mediated SpCas9-LPETG conjugation to V1-ELP at varying ratios. e) Diameter and f) Zeta potential of SpCas9-V1-ELP nanoparticles (n = 3 technical replicates). g) PAGE gel image and h) Densitometry analysis of CD117 mAb-V1-ELP conjugation efficiency. i) MFI and j) Fluorescent images of CD117 + P815 mast cells treated with CD117 mAb-functionalized V1-ELP nanoparticles compared to unfunctionalized controls (n = 3). Scalebars = 10 μm. For all experiments, [V1-ELP] = 8 μM unless otherwise noted. Significance assessed via one-way ANOVA with Dunnett’s correction for multiple comparisons. Data represented by mean ± SEM.
