Fig. 3: Single-cell multiomics readout of functional LNP delivery in NHP.
From: Lipid nanoparticle screening in nonhuman primates with minimal loss of life
a, UMAP clustering of 15 cell types in the livers, with their aVHH protein expression at single-cell resolution, overlaid on the UMAP (78,581 cells from control NHP and 59,082 cells from EoL NHP). BC, B cells; cB/TC, cycling B or T cells; CD4TC, CD4 T cells; CD8TC, CD8 T cells; TC, T cells; KC, Kupffer cells; pDC, plasmacytoid DCs; mDC, myeloid DCs; Mo, monocytes; Neu, neutrophils; Hep, hepatocytes; EC, endothelial cells; Unc, unclassified. b,c, Normalized aVHH expression levels in each cluster from control (b) and EoL (c) livers. The box represents the interquartile range, with the median marked by a horizontal line. d, UMAP clustering of 18 cell types in the spleens, with their aVHH protein expression at single-cell resolution, overlaid on the UMAP (80,893 cells from control NHP and 108,465 cells from EoL NHP). mBC, mature B cells; PreBC, pre B cells; cBC, cycling B cells; NK, natural killer cells; nCD4TC, naive CD4 T cells; Mp, macrophages; Fib, fibroblasts. e,f, Normalized aVHH expression levels in each cluster from control (e) and EoL (f) spleens. The box represents the interquartile range, with the median marked by a horizontal line. g, UMAP clustering of 20 cell types in the bone marrow, with their aVHH protein expression at single-cell resolution, overlaid on the UMAP (78,991 cells from control NHP and 93,780 cells from EoL NHP). PC, plasma cells; Ery, erythroid cells; Gran, granulocytes. h,i, Normalized aVHH expression levels in each cluster from control (h) and EoL (i) bone marrow. The box represents the interquartile range, with the median marked by a horizontal line.
