Fig. 1: RNAcompete-measured RNA sequence specificities.

a, Symmetric heat map displaying PCCs of RNAcompete RNA-binding profiles for each pair of RNAcompete-measured constructs. RBPs are clustered by the AA SID of their RBRs using single-linkage hierarchical clustering. Left: logos of position frequency matrices derived from the top ten RNAcompete 7-mers. Right: eukaryotic clade for each RBP, whether it is newly measured in this study, and the count and class of RBDs in the construct. b, Count of RNAcompete-measured RBPs across major eukaryotic clades for RBPs measured for this study and measured previously. The ‘other’ category encompasses all species outside of metazoa, fungi and land plants, including algae, excavates, amoebozoa, and Stramenopiles, Alveolates and Rhizarians supergroup species. c, Distribution of RNA-binding profile PCCs for pairs of RNAcompete-measured RBPs whose RBRs fall within the AA SID range indicated on the x axis. As a control, the distribution of PCCs between RNAcompete Set A and Set B for the same experiment are displayed to the right. Pairs of RBPs with a PCC of >0.6 (above the red line) are considered to have similar RNA specificities, whereas pairs of RBPs with a PCC of <0.6 have dissimilar RNA specificities. The number of RBP pairs (N) in each AA SID range is indicated above each box. Boxes span the interquartile range (IQR), with the center line marking the median. Whiskers span from minimum to maximum value within IQR × 3 / 2 from box boundaries. Outliers are displayed as dots.