Fig. 3: JPLE predicts RNA-interacting amino acids. | Nature Biotechnology

Fig. 3: JPLE predicts RNA-interacting amino acids.

From: A resource of RNA-binding protein motifs across eukaryotes reveals evolutionary dynamics and gene-regulatory function

Fig. 3

a, The distance between individual amino acid residues and RNA (in angstroms) is compared to JPLE RIS (top) and conservation scores (middle) for the RBP–RNA cocomplex structure in b. RNA-contacting residues (that is, within 5 Å of the RNA) are indicated by dots. A linear visualization of the protein secondary structure is depicted at the bottom. b, The RBP–RNA cocomplex structure (PDB 4ED5) depicts the two N-terminal RRMs from ELAVL1 (Homo sapiens), with regions colored by JPLE RIS. c, Comparison between sequence conservation and JPLE RIS for predicting RRM domain interface residues (that is, the distance from RNA), evaluated with AUROC. Colored circles indicate a significant difference (P < 0.05) in the AUROC between the two scoring methods, as determined by the Delong test (two-sided). Ce, C. elegans; Dm, D. melanogaster; Sc, Saccharomyces cerevisiae.

Back to article page