Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. Can it deliver a new generation of drug targets?
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Change history
07 July 2025
Since the version of the article initially published, Jaimeen Majmudar’s title was amended to “Associate Research Fellow” in the HTML and PDF versions of the article.
References
Liu, Y., Patricelli, M. P. & Cravatt, B. F. Proc. Natl Acad. Sci. USA https://doi.org/10.1073/pnas.96.26.14694 (1999).
Greenbaum, D. et al. Mol. Cell. Proteomics https://doi.org/10.1074/mcp.t100003-mcp200 (2002).
Weerapana, E. et al. Nature https://doi.org/10.1038/nature09472 (2010).
Janes, M. R. et al. Cell https://doi.org/10.1016/j.cell.2018.01.006 (2018).
Baltgalvis, K. A. et al. Nature https://doi.org/10.1038/s41586-024-07318-y (2024).
Klebba, J.E. et al. Preprint at bioRxiv https://doi.org/10.1101/2024.12.17.629001 (2024).
Kavanagh, M. E. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-022-01098-0 (2022).
Vinogradova, E. V. et al. Cell https://doi.org/10.1016/j.cell.2020.07.001 (2020).
Kuljanin, M. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-020-00778-3 (2021).
Parker, C. G. et al. Cell https://doi.org/10.1016/j.cell.2016.12.029 (2017).
Wobst, H. J. et al. JCI Insight https://doi.org/10.1172/jci.insight.182876 (2024).
Bachovchin, D. A., Brown, S. J., Rosen, H. & Cravatt, B. F. Nat. Biotechnol. https://doi.org/10.1038/nbt.1531 (2009).
Johnson, D. S. et al. ACS Med. Chem. Lett. https://doi.org/10.1021/ml100190t (2011).
Rocha, J.-F. et al. Clin. Pharmacol. Ther. https://doi.org/10.1002/cpt.2290 (2022).
Van Esbroeck, A. C. M. et al. Science https://doi.org/10.1126/science.aaf7497 (2017).
Huang, Z. et al. ACS Chem. Biol. https://doi.org/10.1021/acschembio.8b01097 (2019).
D’Souza, D. C. et al. Lancet Psychiatry https://doi.org/10.1016/S2215-0366(18)30427-9 (2018).
Wang, Y. et al. Nat. Chem. https://doi.org/10.1038/s41557-019-0351-5 (2019).
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Garber, K. Probing the proteome. Nat Biotechnol 43, 1216–1220 (2025). https://doi.org/10.1038/s41587-025-02737-2
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DOI: https://doi.org/10.1038/s41587-025-02737-2