Extended Data Fig. 7: Base substitution frequencies at the ADP-ribosylated thymine with DarT2D-nSpCas9 across different targets in EMX1, FANCF and VEGFA genes in U2OS ΔTARG1 cells. | Nature Biotechnology

Extended Data Fig. 7: Base substitution frequencies at the ADP-ribosylated thymine with DarT2D-nSpCas9 across different targets in EMX1, FANCF and VEGFA genes in U2OS ΔTARG1 cells.

From: Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

Extended Data Fig. 7: Base substitution frequencies at the ADP-ribosylated thymine with DarT2D-nSpCas9 across different targets in EMX1, FANCF and VEGFA genes in U2OS ΔTARG1 cells.The alternative text for this image may have been generated using AI.

a, Frequencies of base substitutions at the ADP-ribosylated thymine. b, Locations of base substitutions across the target site. Horizontal bars indicate DarT2 recognition motifs, with ADP-ribosylation of the thymine at the third position. Bars and error bars in a and b represent the mean and s.e.m. of three independent replicates without selection or sorting.

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