Extended Data Fig. 7: Chicken ESCs can contribute to chimera formation.
From: Derivation of embryonic stem cells across avian species
a, Surrogate open-shell system used for the injection of chicken ESCs into chicken embryos. b, Sequential images showing the development and hatchling of a chicken embryo injected with chicken ESCs using the surrogate open-shell system. c, Representative images showing biased lineage contribution in low-rate chicken ESC/embryo chimeras. left panel, E6 chimera (scale bar, 200 μm); right panel, E7 chimera (scale bar, 400 µm). d, The ratios of chimerism in chicken ESC/chicken embryo chimeras generated via non-irradiation and irradiation methods. e, Images showing chimeric chicken embryos across different developmental stages. Chimeric chickens were generated by injecting GFP-positive chicken ESCs (60 passages on feeder plus 15 passages on feeder-free) into EGK.X stage chicken embryos. Scale bars, 500 μm. f, Immunostaining images showing GFP-positive donor chicken ESCs contributing to ectoderm and mesoderm lineages within the chimeric embryo. Top panel, transverse cryosection of the neural tube in a HH10 chimera. Bottom panel, whole-mount staining of the posterior end in a HH5 chimera. Arrows indicate double-positive donor-derived cells. Scale bars, 20 μm. Zoom-in scale bar, 5 μm. Chimeras generated in c, e, f, are from one representative experiment of at least 3 experiments performed.
