Fig. 1: Dual-view light-sheet microscope imaging of preimplantation embryos.
From: Live imaging of late-stage preimplantation human embryos reveals de novo mitotic errors
a, Schematic of the experimental setup: mouse embryos were electroporated with H2B-mCherry mRNA and allowed to recover for 2 h before live-embryo light-sheet imaging. b, Embryos were transferred to the multiposition sample holder. c, Schematic showing the configuration of two detection and two illumination chambers from the top and side views of the microscope sample chamber. The magnified view of the sample chamber displays the illumination beams. d, Mouse embryos expressing H2B-mCherry were imaged by light-sheet microscopy from the blastocyst stage at the selected stages shown. e, Light-sheet imaging of embryos labeled with H2B-mCherry enables visualization of mouse embryo development. Scale bar, 20 µm. f, The duration of initiation of cavitation until blastocyst hatching was quantified in mouse embryos labeled with H2B-mCherry and subject to live-embryo fluorescent light-sheet imaging compared with unlabeled control embryos not subject to light-sheet imaging incubated in conventional conditions. Both groups exhibit similar developmental timing (n = 10 embryos per group). Statistical analysis by two-tailed t-test. NS, not significant. Error bars represent the mean ± s.d. Illustrations in a, c and d created using BioRender.com.
