Extended Data Fig. 5: Micronuclei reincorporation during mitosis and their presence in in vitro cultured and in vivo flushed mouse blastocysts at day 4 post fertilization.
From: Live imaging of late-stage preimplantation human embryos reveals de novo mitotic errors
a, Example of micronuclei reincorporating during mitosis in a human embryo expressing H2B-mCherry. Micronuclei indicated with an arrowhead. Scale bars, 15 μm. Data from three independent experiments b, Representative images of in vitro-cultured and in vivo flushed mouse blastocysts at embryonic day 4 stained for nuclear (DNA, blue) markers. Micronuclei were identified as small, extranuclear DNA structures distinct from the main nucleus (arrowheads). c, Quantification of micronuclei formation in blastocyst-stage embryos. A total of 500 cells from multiple embryos were analyzed. Data are presented as a pie chart, showing the percentage of cells containing micronuclei relative to the total number of cells analyzed. Data from two independent experiments. Scale bar, 30 μm.
