Fig. 2: Live-embryo time-lapse imaging of mouse and human preimplantation embryos reveals differences in interphase duration.
From: Live imaging of late-stage preimplantation human embryos reveals de novo mitotic errors
a, Live-embryo time-lapse light-sheet imaging of embryos labeled with H2B-mCherry enables the visualization of nuclei in mouse development. b, Time-lapse images of mitosis (prophase, metaphase, anaphase and telophase) in mouse cells at selected time points. c, Live-embryo time-lapse light-sheet imaging of embryos labeled with H2B-mCherry enables the visualization of nuclei in human embryo development. d, Time-lapse images of mitosis (prophase, metaphase, anaphase and telophase) in human cells at selected time points. Red arrowheads in b and d indicate the daughter cells resulting from mitosis. e, Quantification of the duration of mitosis in polar versus mural trophectoderm cells from mouse and human blastocyst-stage embryos (n = 17 mouse and n = 13 human blastocysts; n = 90 mitotic cells in mouse blastocysts and n = 90 mitotic cells in human blastocysts). f, Quantification of the duration of interphase in polar versus mural trophectoderm cells from mouse and human blastocyst-stage embryos (n = 17 mouse and n = 13 human blastocysts; n = 45 interphase cells in mouse blastocysts and n = 44 interphase cells in human blastocysts). Statistical analysis by two-tailed t-test. ****P < 0.0001. Error bars represent the mean ± s.d. Scale bar, 20 µm. See Supplementary Videos 1 and 2. T, time. Illustrations in b and d created using BioRender.com.
