Fig. 5: Trophectoderm cells are restricted in human blastocysts.
From: Live imaging of late-stage preimplantation human embryos reveals de novo mitotic errors
a, Live-embryo time-lapse imaging and 3D segmentation of H2B-mCherry expression in human embryo 1 at different time points from 5.5 to 6.5 dpf at the selected time points indicated. The fluorescent signals of H2B-mCherry were used to track cells. b, Number of observed nuclei over the imaging period in human embryo 1. c, Selected frames from time-lapse imaging and 3D segmentation of H2B-mCherry expression in human embryo 2. d, Lineage tree of human trophectoderm-labeled cells at the blastocyst stage (human embryo 2). e, Number of observed nuclei over the imaging period in human embryo 2. f, Selected frames from time-lapse imaging and 3D segmentation of H2B-mCherry expression in human embryo 3. g, Lineage tree of human trophectoderm-labeled cells at the blastocyst stage (human embryo 3). h, Number of observed nuclei over the imaging period in human embryo 3. TE, trophectoderm. Scale bar, 30 µm.
