Fig. 2: Flat-field-corrected light sheet enhances FOV and quality across the imaging tiles.

a, Schematic comparison of using the flat mirror and curved mirror in the ASLM unit and their effect on the field curvature of the light sheet. b, Measured field curvature of the light sheet without (red) and with correction (green). c,d, PSF measurement with 200-nm nanoparticles and comparison without and with the field curvature correction in lateral and axial directions. The PSF FWHM (± s.e.m.) was measured for seven beads in each FOV section. e, 3D rendering and middle slices of the lateral and axial views of the recorded data from a cleared mouse brain, stained for microvessels with three tiles along the height of the light sheet. The images are examples from one of three imaged mouse brains. f, Axial view of the recorded data in d along the light-sheet height and the corresponding Fourier domain comparison with its associated cut of frequency for seven regions. g, Enlarged view of selected areas in f located between adjacent tiles. The arrowheads show the improvements in vessel information with field curvature correction.