Extended Data Fig. 4: Viral RNA stability enhancers act through TENT4-dependent mixed tailing.
From: RNA stability enhancers for durable base-modified mRNA therapeutics
a, Poly(A) tail length analysis by nanopore DRS after transfecting 320 ng of electroporated IVT mRNAs (Control, A2, and A7) into HCT116 cells cultured in 12-wells. Poly(A) tail distributions for control (Ctrl), A2, and A7 element-containing mRNAs ± RG7834 treatment at 12-h post-transfection. The IVT mRNAs originally have a poly(A) tail of 60 nt. Distributions are shown as smoothed histograms to reflect total read count. White lines indicate median values; read counts are shown below. b, Detection of changes in mixed-tailing by direct RNA nanopore sequencing with 320 ng of pooled IVT mRNAs (Control, A2, and A7) same as Extended Data Fig. 3d.
