Supplementary Figure 3: Experimental design and validation of coexpression of GPR88* and mCherry | Nature Genetics

Supplementary Figure 3: Experimental design and validation of coexpression of GPR88* and mCherry

From: Subcellular localization of MC4R with ADCY3 at neuronal primary cilia underlies a common pathway for genetic predisposition to obesity

Supplementary Figure 3: Experimental design and validation of coexpression of GPR88* and mCherry

a, b Design of DIO AAV-expressing Flag-GPR88* or mCherry, stereotaxically injected in experimental a and control b mice. c Coronal section of the PVN of the hypothalamus of Sim1-Cre mice injected with the AAV DIO Flag-GPR88* + AAV DIO mCherry allows for verification of the accuracy and PVN coverage of the injections. d–e Maximal intensity projections of confocal sections through the PVN region indicated by a red square in c show that all mCherry expressing neurons also express Flag-GPR88* (green). White scale bars represent 10 µm.

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