Supplementary Figure 8: TET3 expression in oocytes from ND and HFD mice.
From: Embryonic defects induced by maternal obesity in mice derive from Stella insufficiency in oocytes
a, The relative levels of Tet3 mRNA in MII oocytes obtained from ND and HFD mice were determined by real-time RT–PCR, with Gapdh as an internal control. Data are expressed as the means ± s.d. from three independent experiments. Student’s t test (two-tailed) was used for statistical analysis. Center values, mean; n.s., not significant. b, TET3 protein levels in MII oocytes from ND and HFD mice were evaluated by western blot, with actin as a loading control. The experiments were repeated three times independently with similar results. Band intensity was calculated using ImageJ software, and the ratio of TET3/actin expression was normalized and values are indicated.
