Supplementary Figure 10: Knockdown of endogenous TET3 protein in MII oocytes.
From: Embryonic defects induced by maternal obesity in mice derive from Stella insufficiency in oocytes
Specifically designed siRNAs for TET3 were microinjected into MII oocytes. Samples (100 oocytes per lane) were processed for western blot analysis; actin was served as a loading control. The experiments were repeated three times independently with similar results. Band intensity was calculated using ImageJ software, and the ratio of TET3/actin expression was normalized and values are indicated. Images have been cropped; please compare to Supplementary Data.
