Supplementary Figure 11: TET3-dependent 5hmc and γ-H2AX accumulation in HFD zygotes contributes to early embryonic developmental defects.
From: Embryonic defects induced by maternal obesity in mice derive from Stella insufficiency in oocytes
a, Representative images of 5mC (red) and 5hmC (green) staining on ND zygotes, ND + DMOG zygotes, HFD zygotes and HFD + DMOG zygotes. b,c, Quantification of the levels of 5hmC and 5mC signal in the paternal and maternal pronuclei of zygote (n = 10 biologically independent zygotes for each group). d, Representative images of γ-H2AX (green) and nuclear (red) staining on ND zygotes, ND + DMOG zygotes, HFD zygotes and HFD + DMOG zygotes. e, Quantification of the numbers of γ-H2AX foci in maternal and paternal pronuclei. ♂ and ♀ indicate the paternal and maternal pronucleus, respectively. PB, polar body. Scale bars, 25 μm. Each data point represents a zygote (n = 12 biologically independent zygotes for each group). f, The percentage of zygotes that successfully progressed to the blastocyst stage during in vitro culture. Data are presented as the means ± s.d. from three independent experiments (total number of embryos analyzed: n = 90 for ND, n = 112 for ND + DMOG, n = 95 for HFD and n = 92 for HFD + DMOG). In e and f, statistical analyses were performed with one-way ANOVA with Tukey’s post hoc test. Adjustments were made for multiple comparisons. In all other panels, Student’s t test (two-tailed) was used for statistical analysis. Error bars, s.d.; center values, mean; n.s., not significant.
