Supplementary Figure 15: Effects of Stella overexpression on the methylation status of zygotes in HFD mice.
From: Embryonic defects induced by maternal obesity in mice derive from Stella insufficiency in oocytes
a,c,e,g, Graphical representation of the methylation pattern at the example loci of RLTR10-int, Gab1, Gstp1 and Park2 in ND (top, blue) and HFD (bottom, red) zygotes. The regions highlighted by green boxes were chosen for further validation. b,d,f,h, Bisulfite sequencing for verifying the methylation status of RLTR10-int, Gab1, Gstp1 and Park2 in ND zygotes, HFD zygotes, and HFD zygotes injected with PBS or Stella mRNA. Open and filled circles represent unmethylated and methylated CpGs, respectively. The percentages of methylated CpGs are shown below each panel. The experiments were repeated three times independently with similar results.
